法尼酯X受体对肝脂酶表达的影响

Effects of Farnesoid X Receptor on the Expression of Hepatic Lipase

目的:探讨法尼酯X受体(farnesoid X receptor,FXR)对肝脂酶(hepatic lipase,HL)表达的影响。方法:用FXR激动剂CDCA作用人肝癌细胞株(HepG2),用RT-PCR和Western blotting检测HL的表达情况。结果:用不同浓度的CDCA(25μmol/L、50μmol/L、75μmol/L)分别作用HepG2细胞6h、12h、24h、48h后,HL的mRNA和蛋白质水平呈时间和剂量依赖性下调。结论:FXR激动剂可抑制肝脂酶的表达。

Objective： To investigate the effect of the farnesoid X receptor on hepatic lipase expression. Methods： Human hepatoblastoma HepG2 cells were treated with chenodeoxycholic acid and the levels of HL messenger RNA and protein were determined by RT-PCR and Western blotting respectively, Results： HepG2 cells were treated with FXR agonists CDCA with different concentrations of 25μmol/L, 50μ mol/L, 75μmol/L for 6h,12h,24h and 48h, and the levels of ilL mRNA and protein were decreased in a dose and time-dependent manner. Conclusions： FXR agonist decreases hepatic lipase gene expression.