食管鳞状细胞癌癌组织中hMLH1、hMSH2的表达及其意义

Expression and Clinical Significance of hMLH1,hMSH2 in Esophagus Squamous Cancer

目的:探讨错配修复基因hMSH2、hMLH1在食管鳞癌组织中的表达及其临床意义。方法:采用sP免疫组化的方法检测144例食管鳞癌手术切除标本、30例正常粘膜组织中hMSH2、hMLH1表达情况,并与临床病理参数作相关分析。结果:食管鳞癌组织hMSH2、hMLH1基因阳性表达明显下降,与正常食道粘膜相比相差显著(P<0.01),hMSH2、hMLH1表达缺失与年龄、性别、肿瘤大小、淋巴结转移、浸润深度无显著相关,与肿瘤的分化程度有关(P<0.01)。结论:提示hMLH1和hMSH2基因突变所致的蛋白表达异常缺失与食管鳞状细胞癌发生有关,可能为一种早期事件。

Objective： To investigate the expression of mismatch repair gene hMLH1 and hMSH2 in esophageal squamous cancer and its clinical significance, Methods： We detected the expression ofhMLH1 and hMSH2 respectively in esophageal carcinoma from 144 patients and 30 normal mucosa tissues by SP immunohistochemistry and correlation analysis with clinical patho-parameters, Results： The positive rate of hMLH1 and hMSH2 expression in esophageal squamous cancer tissues was significantly lower than that in normal mucosa tissues, The expression ofhMLHl and hMSH2 protein was closely related to differentiation degree of the cancer, but not obviously to age, gender, lymph node metastasis, tumor size and depth of invasion. Conclusions： It shows that the disfigurement of hMLH1 and hMSH2 expression resulted from gene mutation may be related to esophageal squamous carcinogenesis, and it may be an early event.