生姜石油醚提取物对四种氧自由基体系抗氧化作用的研究

Antiactive oxygen effect of Zingiber officinale petroleum extracts with a four oxygen free radicals system

目的：研究生姜石油醚提取物对４种氧自由基体系的抗氧化作用。方法：用ＵＶ分光光度法研究提取物抗Ｏ２对红细胞（ＲＢＣ）的氧化作用；荧光偏振法测定红细胞膜流动性，研究提取物对红细胞膜的保护作用；荧光法研究提取物对·ＯＨ的抑制率、清除率和抗脂质过氧化作用。结果：提取物有明显的抗Ｏ２对ＲＢＣ的氧化作用。对红细胞膜有保护作用，增加膜的流动性。对·ＯＨ有很强的清除作用，在ｃ＝２．０９ｇ·Ｌ－１时，抑制率和清除率分别为９２．４１％和９７．３９％；并能抑制小鼠肝微粒体的脂质过氧化作用，在ｃ＝４．０ｇ·Ｌ－１时，抑制率为８６．８１％。结论：生姜石油醚提取物对４种不同氧自由基有十分显著的抑制和清除作用，是一种高效的抗氧化剂。

OBJECTIVE: To study antioxidative action of Zingiber officinales petroleum extracts with a four oxygen free radicals system. METHODS: The antioxidative action on erythrocyte with O NFDA2 2 of extracts was studied by spectrophotometry. The protective action on the treatment of erythrocyte membranes with H 2O 2 and the cell membrane fluidity with fluorescence polarization were studied.The rate of inhibition and scavenging of ·OH generation and the antiaction of lipid peroxidant of zingiber extracts were studied by using fluorescence. RESULTS: The zingiber extracts could significantly antioxidative action on erythrocyte with O NFDA2 2, and had protective action on erythrocyte membranes and caused an increase in fluidity. The extracts possessed great scavenging effect on ·OH, the rate of inhibition and scavenging were 92.41% and 97.39% respectively when c=2.09 g·L -1 , and the lipid peroxidation of mouse liver mitochondria was markedly inhibited by extracts at c=4.0 g·L -1 . The rate of inhibition was 86.81%. CONCLUSION: The extracts of zingiber significant inhibition and scavenging effect on four oxygen free radicals, and can be used as an antioxidant.