4-1BB配体在肿瘤细胞株上的表达及其与肿瘤细胞增殖的关系

The Relationship between the Expression of 4-1BBL and the Proliferation of Tumor Cell Lines

目的检测4-1BBL在肿瘤细胞株上的表达及其功能。方法半定量RT-PCR检测几种肿瘤细胞株内4-1BBL mRNA表达;流式细胞术检测肿瘤细胞表面4-1BBL蛋白的表达;MTT法检测4-1BBLmAb对肿瘤细胞增殖的影响。结果4- 1BBL在Raji和THP-1细胞株表面表达率分别为26.8%和15.3%,而在T1301和Jurkat细胞株中,4-1BBL mRNA水平虽有一定表达,但蛋白水平却几乎不表达;4-1BBLmAb可以促进肿瘤细胞的增殖。结论4-1BBL在不同肿瘤细胞株上的表达有差异;4-1BBL在肿瘤细胞的增殖和长期存活中发挥重要作用。

Objective To detect the expression and the function of 4-1BB ligand on tumor cell lines. Methods The expression level of 4-1BB ligand mRNA and protein on tumor cell lines was detected by quantitive RT-PCR and FACS. Using 4-1BBL monoclonal antibody（mAb） to stimulate the tumor cell lines, and the effect of proliferation of the tumor cell lines through 4-1BB LmAb was detected by MTT methods. Results The 4-1BB ligand mRNA was detected in Rail, THP-1, Jurkat and T1301, but only Raji and THP-1 could express 4-1BB ligand protein molecules accordingly（26.8% and 15.3%, respectively）. 4-1BBLmAb could enhance the proliferation and survival in the tumor cell lines. Conclusions The expression levels of 4-1BB ligand on different tumor cell lines are different and may be related to their rapid growth. The 4-1BB ligand plays an important role in the proliferation and survival of tumor cell lines.