大白菜新型细胞质雄性不育生理生化机制的研究

Studies on Physiological and Biochemical Mechanisms for Cytoplasmic Male Sterility in Chinese Cabbage

以大白菜新型细胞质雄性不育系及其保持系为材料,对花蕾发育全过程中氨基酸、可溶性糖、蛋白质含量及同工酶谱带进行了动态比较研究。结果表明:不育系花药中可溶性糖、可溶性蛋白质含量低于其相应的保持系;其游离氨基酸总量和保持系无明显差异,但不育系的脯氨酸含量显著低于其相应的保持系;不育系花药中MDH,AAT,EST,POD,COD和PPO同工酶谱带与保持系相比存在明显差异。认为不育系花药中结构物质、营养物质、能量物质的亏损,同工酶表达的差异与小孢子败育密切相关。

CMS is one of the most important system for F1 seed production in Chinese cabbage. Concerning the physiological and biochemical mechanisms of CMS, the changes of soluble sugar, soluble protein, amino acids and isozymes in anther at different development stage of Cytoplasmic Male Sterility line and its maintainers in Chinese cabbage have been investigated in this study. It showed that the contents of soluble sugar, soluble protein in CMS were significant lower than its maintainer. No difference was observed in the contents of total free amino acids between CMS and its maintainer. However, the proline content in CMS was significantly lower than its maintainer; Bands pattern differences were observed for 6 kinds of isozymes （AAT, MDH, EST, POD, COD and PPO） in anther at different development stages between sterile and maintainer line.