睡眠剥夺对大鼠氧惊厥的影响

The effect of sleep deprivation on hyperbaric oxygen-induced convulsion in rats

目的观察睡眠剥夺对大鼠氧惊厥的影响。方法采用小站台水环境睡眠剥夺模型,大鼠睡眠剥夺24h、48h、72h后高压氧暴露,记录氧惊厥潜伏期及氧惊厥严重程度(SOC)计分。结果大鼠睡眠剥夺各后组氧惊厥潜伏期小于氧惊厥组,且睡眠剥夺时间愈长潜伏期愈短,72h组(15.7±5.6)min<48h组(17.2±3.5)min<24h组(20.5±3.2)min(P<0.05),大鼠睡眠剥夺各组氧惊厥的SOC计分高于氧惊厥组,且睡眠剥夺时间愈长SOC计分愈高,72h组(98±4)分>48h组(83±6)分>24h组(74±5)分(P<0.01)。结论睡眠剥夺可以降低氧惊厥发作的阈值,加重氧惊厥的程度。

Objective To observe the effect of sleep deprivation （SD） on hyperbaric oxygen-induced convulsion （HBOC） in rats. Metods Power pot technique was used to deprive the rapid-eye movement sleep of rats for 24 h,48 h and 72 h respectively. Then the rats were exposed to hyperbaric oxygen. Latency period and severity of convulsion （SOC） of hyperbaric oxygen-induced convulsion were recorded. Results The latency periods of HBOC in SD 24 h,48 h and 72 h groups were significantly shorter than those in HBOC group, and which was （20.5 ±3.2）min in SD 24 h group,（17.2±3.5）min in 48 h group,（15.7 ±5.6）min in SD 72 h group （ P 〈 0.05）,SOC in SD 24 h, 48 h and 72 h groups were significantly higher than those in HBOC group. The SOC scores were the highest in SD 72 h group （98 ±4）, the medium in SD 48 1 group（83±6）, the lowest in SD 24h group（74 ± 5）（ P 〈 0.01 ）. Conelusion SD could reduce the attack threshold of HBOC and could aggravate the severe degree of HBOC.