摘要
利用快提法对不同取样部位及不同取样时期的样品提取DNA并进行PCR检测，结果表明：PCR扩增效果较好的样品包括种子胚、胚乳、未发芽时的水培初生根和次生根的根尖、发芽1d的胚芽（幼叶）、发芽1—3d的胚芽鞘、以及发芽3．5d的中胚轴；而发芽后的水培根尖、发芽3d的幼叶、发芽5d的胚芽鞘、发芽10d的中胚轴提取的DNA扩增效果较差或没有扩增产物。
Different tissues and the optimal time fitting for quick DNA extraction technique were compared. The results showed that the technique was well applied in the following tissues for DNA extraction： embryo, endosperm, radicel cultivated in water,coleoptile germinated 1 -3 d, hypocotyl germinated 3 -5 d materials. But the DNA extracted from tender leaf, eoleoptile, radicel missing optimal time was failed amplification.
出处
《华北农学报》
CSCD
北大核心
2007年第5期114-116,共3页
Acta Agriculturae Boreali-Sinica
基金
北京农业育种基础研究创新平台
北京市自然科学基金项目(YZPT02-06)
关键词
DNA快速提取
玉米
胚
胚乳
Quick DNA extraction
Maize
Embryo
Endosperm
