元宝草悬浮细胞培养及其生长特性研究

Cell Suspension Culture and Growth Characteristics of Hypericum sampsonii Hance

研究了元宝草悬浮细胞生长及其金丝桃素类物质（金丝桃素和假金丝桃素）的代谢规律。悬浮培养细胞的生长周期为18d，其中4～10d是对数期。金丝桃素类物质在10d达到46．06μg／g（DW），其含量增加与细胞鲜质量增长相偶联。可溶性糖的消耗与细胞的生长以及金丝桃素类物质的代谢有着密切的关系，是影响细胞鲜质量和金丝桃素类物质增长的重要因素。

This research studied the cell growth characteristics and hypericins （hypericin and pseudohypericin） metabolite production in Hypericum sampsonii Hance cell suspension culture. The result as followed ： growth period of suspension cell was as long as 18 d;the exponential phase was 4 - 10 d. Hypericins arrived 46.06 ttg/g（DW）at 10 day＇s culture, and its production was related to cell growth rate. Consume of soluble carbohydrate that played a key role in cell growth and hypericins metabolism, effected accumulation of fresh weight of cell and hypericins.