油菜内生细菌yc8促生作用及其机理研究

Studies on the Growth Promotion of Endopytic Bacteria yc8 from Rape

从山西太谷采集的油菜中分离到具有生防潜力的菌株yc8,经鉴定该菌株属于环状芽孢杆菌(B.circulans)。并测定了该菌株发酵液对不同油菜种子萌发、胚根及胚轴生长的影响,及油菜萌发中α-淀粉酶活性和(α+β)-淀粉酶总活性变化。结果表明:油菜内生细菌yc8发酵液稀释100倍浸种处理,对不同油菜种子萌发率、胚根及胚轴生长影响最大,与其他浓度的处理相比差异显著;对不同油菜品种之间的促生作用有一定的差异。并且发酵液稀释100倍浸种处理时,不用油菜品种α-淀粉酶的酶活力最高,分别是各自对照的0.93和0.59倍。而β-淀粉酶的酶活力变化却不明显。(α+β)-淀粉酶的酶活力与α-淀粉酶的酶活力成正相关。

By the primary antagonistic screening study, inhibitory activity of yc8 which endophytic bactenas strain was isolated from rape collected from Taigu in Shanxi province was obvious. Though primary taxonomy study, it is identified to be Bacillus. circulans. This experiment studied the different fermented fluid concentrations of endopytic bacteria yc8 from rape on effect to the sprout rate,the radicle and the germ,and determined the variance of α-amylase,β-amylase and total amylase activity in the sprout between various kind of rape. The result indicated the 100-fold fermented fluid concentration affected the sprout rate, the radicle and the germ much more. Compared with other concentration, it had significant difference. And α-amylase activity was the highest in the steep treatment of 100-fold fermentable production,0.93 fold and 0. 59 fold compared with the contrast respectively, but the change of β-amylase was not obvious.