芬兰虹鳟鱼同工酶分析

Study On The Isozymes In Jalo Rainbow Trout(Oncorhynchus Mykiss)

运用聚丙烯酰胺凝胶电泳法对芬兰虹鳟肌肉(M)、心(H)、肝(L)、肾(K)、眼(E)等5种组织中的乳酸脱氢酶(LDH)、乙醇脱氢酶(ADH)、苹果酸酶(ME)、葡萄糖-6-磷酸脱氢酶(G6PDH)、过氧化物酶(POD)和酯酶(EST)进行了初步研究,并对各种酶的同工酶位点及酶谱表型进行分析。结果表明,除酯酶外,其他5种同工酶均表现出不同程度的组织特异性。同工酶表型种群遗传学研究发现,6种同工酶共记录15个基因座位,多态座位比例(P=40%)略高,位点有效等位基因数(Ne=1.4)较大,表明芬兰虹鳟种群遗传多样性偏高。

Five tissues （ muscle, heart, liver, kidney, and eye） of Jalo rainbow trout （ Oncorhynchus mykiss） were used to investigate six kinds of isozymes （ LDH, ADH, ME, G6PDH, POD and EST） by means of polyacrylamide gel electrophoresis. Result showed that all the isozymes have clearly tissue specificity expect EST. Biochemical genetic analysis proved 6 isozymes were coded by 15gene loci. The percentage of polymorphic loci is 40% and the average value is 1.4. Both of the two indexes are high, which indicates that the population genetic diversity of Jalo rainbow trout （Oncorhynchus mykiss） is prominent.