内皮抑素的低分子肝素修饰及对兔角膜新生血管的抑制作用

Modification of endostatin with low molecular weight heparin and its angiogenesis effects on rabbit corneas

目的探讨利用低分子肝素对内皮抑素进行结构修饰,并评价其对角膜新生血管(corneal neovas-cularization,CNV)的抑制作用。方法采用化学方法用低分子肝素修饰内皮抑素,碱烧伤法建立兔角膜新生血管模型,分别在球结膜下注射低分子肝素化内皮抑素(Ⅰ组)、内皮抑素(Ⅱ组)和生理盐水(对照组),观察新生血管的生长情况。结果成功的利用低分子肝素对内皮抑素进行了结构修饰,动物实验表明Ⅰ组新生血管的长度、面积、数量、VEGF的表达均明显低于其它两组,差异有统计学意义(P<0.05)。结论低分子肝素化内皮抑素可有效抑制角膜新生血管的生长,效果优于内皮抑素。

Objective To investigate the modification of endostatin （ES） with low molecular weight heparin （LMEH） and its inhibitory effects on corneal neovascularization（CNV）. Methods ES was modified with LMWH by a chemical method. The rabbit CNV model was made with alkali burning. LMWH-ES（group 1）, ES （group 2） and normal saline（control group） were subconjunctivally injected. Results The blood vessel was shorter and smaller in group 1 than in the other two groups（ P 〈 0.05） and the expression of VEGF in group 1 was also significantly lower than that of the other two groups（ P 〈 0.05）. Conclusions LMWH-ES can effectively inhibit the growth of CNV and is superior to ES.