摘要
双孢蘑菇子实体（Ａｇａｒｉｃｕｓｂｉｓｐｏｒｕｓ）经热水提取，乙醇沉淀，胰蛋白酶消化，Ｓｅｖａｇ法去除蛋白质等处理，得多糖。经紫外可见光分光光度计测定无紫外吸收峰，为较纯多糖。并研究了其对人肝癌ＳＭＭＣ－７７２１细胞生长曲线、有丝分裂指数及线粒体活性的影响，结果表明该多糖对体外培养的人肝癌ＳＭＭＣ－７７２１细胞增殖有一定抑制作用，抑癌实验证明该多糖对小鼠Ｓ－１８０实体瘤亦有一定抑制作用。
The polysacchride was isolated from the fruit bodies of Agaricus bisporus Sing. and verified by ultraviolet spectrum. The proliferation of human hepatoma SMMC-7721 cell line was significantly inhibited by the treatment of 50ug/mL the polysacchride. The conclusion was proved by determining the variation of the growth curve, mitosis index and mitochodria activity. The inhibitory activity of the polysacchride against implanted S-180 tumor in mice was also perfromed with the 51.9% inhibition.
出处
《中国食用菌》
1997年第4期5-7,共3页
Edible Fungi of China
基金
山东省科委资助
