摘要
灵芝菌丝体超氧化物歧化酶（ＳＯＤ）同工酶谱带有５条，其中酶带Ⅰ，Ⅳ，Ⅴ为Ｍｎ—ＳＯＤ，酶带Ⅱ，Ⅲ为Ｃｕ—Ｚｎ—ＳＯＤ。Ｃｕ—Ｚｎ—ＳＯＤ在不同浓度的ＫＣＮ作用下，其活力随ＫＣＮ的浓度增大而逐渐丧失，而Ｍｎ—ＳＯＤ不受ＫＣＮ影响仍具催化活性；Ｃｕ—Ｚｎ—ＳＯＤ在氯仿—乙醇的作用下仍具催化活性，而Ｍｎ—ＳＯＤ随氯仿—乙醇的浓度增大而失去活力；ＳＤＳ可使Ｍｎ—ＳＯＤ失去活性，但对Ｃｕ—Ｚｎ—ＳＯＤ无影响；β—巯基乙醇使部分Ｃｕ—Ｚｎ—ＳＯＤ失去活性，但对Ｍｎ—ＳＯＤ无影响。
In five isoenzyme bands of SOD in mycelium of Ganoderma lucidum band Ⅰ,Ⅳ,Ⅴ are Mn-SOD and band Ⅱ,Ⅲ are Cu-Zn-SOD. The activity of Cu-Zn-SOD decreases with the improvement of KCN content and β-mercaptoethanol and it is not effected by chloroform-ethanol and SDS. Meantime, the activity of Mn-SOD decreases with the improvement of chloroform-ethanol and SDS, and it is not effected by KCN and β-mercaptoethanol.
出处
《中国食用菌》
1997年第4期32-34,共3页
Edible Fungi of China
