摘要
目的:探讨Bcl-2基因的RNA干扰(RNA interference,RNAi)表达载体对卵巢癌SKOV3细胞Bcl-2基因表达的抑制作用。方法:构建针对Bcl-2基因的RNAi表达载体,脂质体法转染卵巢癌SKOV3细胞株,应用RT-PCR及流式细胞仪检测其对Bcl-2基因mRNA及蛋白表达的影响。结果:构建的两种表达载体均抑制了Bcl-2基因的mRNA及蛋白的表达,其中转染2号载体的SKOV3细胞Bcl-2基因mRNA表达仅相当于对照组的32.3%,蛋白抑制率达60.18%。结论:RNAi表达载体可以有效抑制Bcl-2基因的表达。
Objective： To investigate the inhibitory effects of RNAi expression vector of Bcl - 2 gene on Bcl - 2 gene expression of SKOV3 cell of ovarian carcinoma. Methods： RNAi expression vector for Bc1-2 gene was constructed and transfected to SKOV3 cell strain of ovarian carcinoma with llposomes. RT - PCR and flow cytometer detects effect to mRNA and protein expression of Bcl - 2. Results： The two kinds of constructive expression vectors inhibited both mRNA and protein expression of Bcl -2. Among Bc1-2 mRNA and protein expression of SKOV3 cell that transfected B type carrier only equaled to 32.3% of contrastive groups. The anti - rate of protein was 60. 18% . Conclusion： The RNAi expression vector inhibits effectually expression of Bcl -2 gene.
出处
《中国妇幼保健》
CAS
北大核心
2007年第32期4594-4596,共3页
Maternal and Child Health Care of China
基金
重庆市自然科学基金(CSTC2004BB5123)
