鼻咽癌的基因不稳定性

Genomic instability in nasopharyngeal carcinoma

目的：探讨鼻咽癌基因不稳定性对预后的意义。方法：运用单一重复序列间多聚酶链反应（inter-SSRPCR）对38位鼻咽癌患者基因不稳定性进行评估;运用测序和巢式PCR对不同肿瘤的突变片段进行检测。结果：38位患者中有31位（81.6%）显示了基因组失稳,其失稳指数范围为0%～16.2%。6个肿瘤的6q27染色体上证实有获得性基因损害。低于5年生存期患者其基因失稳程度显著高于超过5年生存期的患者（P〈0.05）。结论：基因组失稳可作为鼻咽癌发生的早期标志,并且基因组失稳程度加重提示肿瘤预后不良。

Objective To evaluate the effect of genomic instability on prognostics in nasopha ryngeal carcinoma. Methods Genomic instability was assessed by inter-simple sequence repeats polymerase chain reaction （inter-SSR PCR） in 38 patients with nasopharyngeal carcinoma. Characterization and verification of band alterations shared in different tumors were carried out by sequencing and nest PCR. Results Thirty- one （ 81.6 % ） of the 38 patients showed genomic alteractions, and genomic instability index ranged 0 to 16. 2 percent. A gain-based genomic damage shared in 6 tumors was identified on chromosome 6 q27. Genomic alteration was significantly more in patients less than 5-year survival than those with more than 5-year survival （ P 〈 0.05 ）. Conclusion Genomic instability can be an early event marker in carcinogenesis of nasopharyngeal carcinoma. Aggravation of genomic alterations is a poor prognosis for cancer recoverv.