铁(Ⅲ)与脱氧核糖核酸作用的共振瑞利散射光谱研究

Resonance Rayleigh Scattering Study of the Interaction of Iron(Ⅲ) with Deoxyribonucleic Acid

研究了Fe^3＋与脱氧核糖核酸（DNA）的共振瑞利散射（RRS）光谱和适宜的反应条件。实验结果表明，在pH2．20的Britton—Robinson缓冲溶液中，Fe^3＋与DNA自身的共振瑞利散射峰均较弱，但当二者发生静电作用形成缔合物后，体系的共振光散射峰增强，最大散射峰位于320．0nm处。当Fe^3＋浓度为2．75×10^-3 mol／L时，在0—8．4mg／L范围内，共振光散射强度与DNA的浓度呈线性关系。检出限为2．60μg/L。据此建立了一种定量测定DNA的简便、快速的新方法。本方法用于合成样品中痕量DNA的测定，回收率为93％～101％．

A novel method based on the enhanced resonance Rayleigh scattering （RRS） of Fe （ Ⅲ） - DNA complex in Britton - Robinson buffer solution of pH 2.20 was proposed for the determination of DNA. The RRS intensity was measured at A320 nm, the maximum scattering wavelength of Fe （ Ⅲ ） - DNA complex. The characteristics of RRS spectra of Fe （ Ⅲ ） - DNA complex, the effects of pH, Fe （ Ⅲ ） concentration, the sequence of adding the reagents and the coexistent substances were studied. The RRS intensity was directly proportional to the concentration of DNA in the range of 0.0 - 8.4 mg/L with a detection limit of 2.60 μg/L. The method has been applied to the determination of DNA in synthetic samples with a recovery range of 93% - 101%.