静电场对小鼠体外受精胚胎质量的影响

The Research of the Spatial Pattern Population in Montane Tropical Rain Forest in Wuzhi Moutain,Hainan

利用静电场处理体外受精（ＩＶＦ）小鼠原核期胚胎，并在体外进行发育培养．在此过程中通过观察ＩＶＦ胚胎的发育速度及冷冻－解冻后囊胚孵化率，探讨了静电场对小鼠ＩＶＦ胚胎质量的影响．实验结果显示，授精处理后８４ｈ，处理组的囊胚出现率（４６．５％）显著高于对照组（２５．５％）（Ｐ＜０．０５），并且到１０８ｈ时，处理组的囊胚孵化率（９４．４％）也显著高于对照组（６０．０％）（Ｐ＜０．０１）．表明静电场处理胚胎的发育速度快于对照组．冷冻－解冻实验结果表明，处理组胚胎的形态正常率与对照组无差异，然而囊胚孵化率却高于对照组１８．４％，存在显著差异（Ｐ＜０．０５）．

Spatial patterns of ten major populations in tropical montane rain forest are investigated on Wuzhi moutain,Hainan,through variance/mean ratio,Moristia′s index of dispersion,Modified nearest neighbour technique and Combined countdistance analysis.The previous two methods are based on the individual numbers in the quadrats and the latter two are based on the distance between individuals.All the results show that four species are randomly distributed,the rest six have different distribution patterns with four different methods.