野生大家鼠和实验大白鼠血红蛋白和乳酸脱氢酶同工酶的比较研究

COMPARATIVE STUDIES ON THE HEMOGLOBIN AND LACTATE DEHYDROGENASE ISOZYMES FROM TWO KINDS OF WILD AND LABORATORY RATS

本文对野生大家鼠（Ｒａｔｕｓｎｏｒｖｅｇｉｃｕｓ）和Ｗｉｓｔａｒ品系实验大白鼠的血红蛋白（Ｈｂ）及组织的乳酸脱氢酶（ＬＤＨ）同工酶进行了电泳分析，结果表明，两种鼠均表现了Ｈｂ的多态性，其中ＨｂＩ型都含有８个成分，各成分的泳动率相同。两种鼠的心脏ＬＤＨ同工酶都含有ＬＤＨ１，ＬＤＨ２，ＬＤＨ３和ＬＤＨ５，而且各谱带泳动率相近似，其余组织ＬＤＨ谱带泳动率有明显的差异，表现了组织和种（或品系）的特异性。从电泳图谱上发现Ｗｉｓｔａｒ大鼠ＬＤＨ５（Ａ或Ｍ亚基纯合体）出现亚带，而ＬＤＨ１（Ｂ或Ｈ亚基纯合体）则未发现，可确定变异是由于Ａ或Ｍ亚基位点发生突变，出现等位基因而导致。

The hemoglobins and lactate dehydrogenase(LDH)isozymes in wild rat ( Rattus norvegicus ) and wistar strain of laboratory rat have been analysed by means of polyacrylamide gel disc electrophoresis. All animals adult. The results are as bellows: 1. The biochemical polymorphism of the hemoglobin was found in two kinds of wild and laboratory rats,Rattus norvegicus has 3, 6, 7 and 8 components; wistar strain rat has only 8 components.Hemoglobin type Ⅰof both rats possessed eight zones and their migration rates(Plate Ⅰand Fig 1) and scanning curves(Fig 2)were similar. 2.Each tissue LDH isozyme in Rattus norvegicus and wistar strain rat was found to have a characteristic distribution of these isozymes in the pattern, but the isozyme bands in heart have appeared LDH1,LDH2,LDH3 and LDH5 and migration rates were similar , in other tissue the numder and the mobility of LDH iszoymes were different(PlateⅡ). 3. The electrophoretic variants of serum and tissue LDH isozyme from wistar strain are described, since sub band have appeared within LDH5 and LDH4, it indicates that LDH A( M)locus occurs in mutant alleles.