蝙幅葛苏林碱对大鼠复灌性局部脑缺血病理变化的影响

The effect of daurisoline on pathologcial changes of regional cerebral ischemia-reperfusion in rat

目的探讨蝙幅葛苏林碱（DSL）对局部脑缺血再灌注损伤的保护作用。方法32只Wiistar雄性大鼠随机分为4组。．Ⅰ．Ⅱ．Ⅲ组分别为阻断大脑中动脉（MCA）后立即腹腔注射DSI·2mg·kg－1、5mg·kg-1和8mg·kg-1的实验组；IV组为阴性对照组。建立大鼠复灌性局部脑缺血模型，观察阻断大脑中动脉3h．复通21h的双侧脑组织的病理变化。结果未阻塞测未观察到脑组织的病理变化。阻塞侧，Ⅱ、Ⅲ组病理变化相似，Ⅰ、Ⅳ组病理变化相似，但是Ⅰ、Ⅳ组明显重于Ⅱ．Ⅲ组。结论提示DSL对复灌性脑缺血损伤有保护作用。

Objective To research the protective effedts of the daurisoline on injury of the focal cerebral ischemin-reperfusion.Methods Thirty ttvo Wistar rats were divided into four groups randomly. Group 1,group 2 and group 3 were experimental groups which daurisoline ip(2mg. kg-1,5mg' kg-1and 8mg.kg-1respectively), and group 4 was negative control. The model of focal cerebral ischemia-reperfusion was developed in ratS, and meanswhile pathological Changes were observated at 3 hours and reperfuSion 21 hours of both cerebrum.Results No Pathological changes were observed in opposite of the occluded (middfe cerebral aftery,MCA).In side of the occluded MCA,sedr pathological changes of the cerebrum have taken place in the group 2 and group 3, and sitnllar pathologica1 changes of the cerebrum have taken place in the group 1 and group 4, neveftheless,pathological changes of the group 1 and group 4 were more remarkab1e than that of the group 2 and group 3.Conclusion It is concluded that daurisoline have the protective effeCts on the focal injery of cerebral ischemin-reperfusion.