脑康灵胶囊抗大鼠脑缺血的研究

Study on the Effect of Naokangling Capsule against Cerebral Ischemia in Rats

目的观察脑康灵胶囊对脑缺血/再灌注损伤的保护作用,并对其机制作初步探讨。方法采用大鼠大脑中动脉缺血2h/再灌注22h模型,神经病学评分,脑梗塞范围及脑组织水含量变化,观察脑康灵胶囊抗脑缺血/再灌注损伤的效应;通过测定大鼠脑组织中一氧化氮合酶(nitric oxide synthase,NOS),超氧化物歧化酶(superoxide disumtase,SOD)活性及丙二醛(malondialdehyde,MDA)含量的变化以探讨药物作用的机制。结果脑康灵胶囊可降低大鼠脑缺血/再灌注后神经病学评分及梗塞范围,降低脑组织MDA,NOS含量及升高SOD活性。结论脑康灵胶囊对脑缺血/再灌注损伤有保护作用,其作用机制与抑制NOS活性、抗脂质过氧化、提高SOD活性有关。

Objective To observe the protective effects of Naokangling Capsule en cerebral ischemia/reperfusion injury in rats. Methods The rat model of middle cerebral artery ischemia 2h/reperfusion 22h was established. The neurological scale, cerebral infracted volume, cerebral water content, activities of NOS and SOD were measured. Results The average neurological score, cerebral infracted volume, cerebral water content, activity of NOS, content of MDA in Naokangling Capsule group significantly decreased. Meanwhile, the activity of SOD significantly increased compared with control group. Conclusion Naokangling Capsule may protect cerebral ischemia/reperfusion damage, and the mechanism might be related with inhibiting the activity of NOS, lipoperoxidation and increasing the avtivity of SOD.