凉燥致病机制的实验研究

Study on the Pathogenic Mechanism of Mice with Cool-Dry

目的研究凉燥对小鼠的主要致病机制。方法216只无特定病原体级(SPF)昆明小鼠随机分为常温常湿组(A组),常温燥组(B组),凉燥组(C组),每组72只。在模拟凉燥条件下造模,第7天与第14天检测气道与皮肤组织病理、气管纤毛运动、气道液分泌(RS)与气道液IgG抗体(IgG-R)、血液流变学、细菌攻击实验与气管细胞bcl-2基因、bax基因表达。结果与A组相比,C组第14天气管、肺与皮肤病变明显,肺细菌数增高,IgG-R增高,第7天RS降低(P<0.01),血液流变学影响不明显,但气管细胞bcl-2基因表达下降。结论凉燥伤肺、伤津,致气道"纤毛-黏液毯"局部御邪屏障受损;肺津凝滞于肺,以致宣输失司则皮肤失养。本结果为凉燥致病提供实验资料。

Objective To investigate the pathogenic effects of Cool - Dry on mice. Methods 216 Kunming mice（SPF） were divided into three groups including group A（ normal mice）, group B（ normal temperature and Outer-Dry mice） ,group C（ Cool -Dry mice） ,72mice/group. Respiratory secretion of Mucopolysacchride（RS） ,IgG of Respiratory（IgG-R） ,hemorrheology, Bacterial attacking and Expression of bcl-2, bax gene were tested in clay 7 and 14. Results In group C the pathologreal changes of respiratory and skins were obvious, and bacillia quantity of lung increased, bcl-2 gene expression was reduced significantly compared with group A（P 〈0.01 ） . Conclusion It has first provided the evidences of Cool-Dry that injured the windpipe and skins and the defenced of ＂lanket of Cilia - mucus＂ and reduced the expression of bcl - 2 gene, which was related to the mechanisms of Cool-Dry.