铁皮石斛茎节离体培养的研究

In vitro Propagation of Dendrobium candidum from Axenic Nodal Segments

目的通过无菌茎段实现铁皮石斛快速繁殖。方法将铁皮石斛试管苗茎节接种于不同NAA浓度的MS培养基上诱导腋芽萌发,并在含不同激素配比和有机添加物的培养基上继代和诱导生根。结果NAA0.4mg/L,6-BA5mg/L时铁皮石斛腋芽萌发率达93.33%;NAA0.3mg/L,6-BA2mg/L时丛苗的生根率达96.67%,香蕉汁和土豆汁能够显著促进丛生芽的增殖和壮苗生根。结论建立了适合铁皮石斛茎段离体培养的快速繁殖体系。

Objective To develop a rapid propagation of Dendrobium candidum using the aseptic nodal segments of shoots. Methods Axenic nodal segments were first germinated on Murashige and Skoog（MS） basal medium supplemented with different concentration NAA. The seedlings were subcultured and rooted on the medium containing different concentration hormones and organic susbstance. Results 93.33% of the axenic nodal segments of Dendrobium candidum germinated on the medium with NAA 0.4 mg/L,6 - BA 5 mg/L and 96.67% of explant rooted on the medium containing NAA 0.3 mg/L,6 - BA 2 mg/L. The growth of explant was promoted by the extract of banana and potato. Conclusion The in vitro propagation of Dendrobium candidum from axenic nodal segment has been established.