异育彭泽鲫及其双亲消化酶活性的比较研究

COMPARISON OF DIGESTIVE ENZYME ACTIVITY IN DIGESTIVE ORGANS BETWEEN BUMPER CRUCIAN CARP(CARASSIUS AURATUS OF PENGZE♀×CYPAINUS ACUTIDORSALIS ♂) AND ITS PARENTS

对异育彭泽鲫及其双亲肝胰脏和前、中、后肠消化酶活性进行比较研究.结果表明:异育彭泽鲫肝胰脏及前、中、后肠的蛋白酶活性分别为367.69、101.16、297.06、25.97μg/(g.m in),均介于其双亲之间;异育彭泽鲫肝胰脏及前、中、后肠的淀粉酶活性分别为300.60、425.45、272.58、312.28μg/(g.min),均大于双亲;异育彭泽鲫肝胰脏及前、中、后肠的脂肪酶活性分别为1.76、2.42、1.23、1.04μg/(g.min),前肠和中肠脂肪酶活性大于双亲,后肠和肝胰脏脂肪酶活性介于双亲之间,且3种消化酶活性都比母本高.差异性分析结果表明,其与母本之间的差异显著低于其与父本的差异.研究结果符合雌核发育子代表型与母本一致的特性,并显示异源精子对雌核发育子代肝胰脏及肠管道消化酶活性有一定影响,为异育彭泽鲫的生长效应提供了一定的证据.

Abstract： The digestive enzyme activity in digestive organs between Bumper crucian carp （ Carassius auratus of Pengze ♀× Cypainus acutidorsalis ♂ ） and its parents is studied. The results show that the protease activities in liverpancreas and foregut, middlegut, hindgut of Bumper crucian carp are 367.69,101.16,297.06,25.97 μg/（g·min） respectively, all between its parents. The amylase activities in liver - pancreas and foregut, middlegut, hindgut of Bumper crucian carp are 300. 60,425.45,272.58,312. 28 μg/（g·min） respectively, all higher than its parents＇. The lipase activities in liver - pancreas and foregut, middlegut, hindgut of Bumper crucian carp are 1.76,2.42,1.23,1.04 μg/（g·min） respectively. The lipase activities in foregut and middlegut are higher than its parents＇ , but between its parents＇ in liver - pancreas and hindgut. Three digestive enzyme activities in digestive organs of Bumper crucian carp are also higher than its mother＇ s, The difference analyses show that the difference between mother and filial generation is lower than what between father and filial generation. The results accord with the characteristic which the phenotypes of Bumper crucian carp are unanimous with its mother＇ s, and also indicate that heterogeneous sperm have some effects on digestive enzyme activity. This experiment offers the evidence for the growth effect of Bumper crucian carp.