高效液相色谱电化学检测测定血浆中丙咪嗪及其代谢物

DETERMINATION OF IMIPRAMINE AND ITS METABOLITES INPLASMA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHYWITH ELECTROCHEMICAL DETECTION

用高效液相色谱电化学检测法测定血浆中丙咪嗪及其代谢物浓度。以１％三乙胺磷酸缓冲液（ｐＨ３．０）：甲醇：四氢呋喃（６０：３０：１０，ｖ／ｖ）为流动相，在电化学检测器０．７ｖ的工作电压下进行检测。丙咪嗪、去甲丙咪嗪、２－羟基丙咪嗪和２－羟基去甲丙咪嗪的最低检测浓度分别为０．５，０．５，０．６和０．８μｇ／Ｌ血浆，平均萃取回收率分别为８６．９％，８８．４％，８２．５％和７８．８％，日内ＣＶ％分别小于３．９６，４．７２，６．０５和６．６４，日间ＣＶ％分别小于５．６３，５．３３，４．９５和６．２４。方法简便、灵敏，可充分满足临床药理研究和治疗药物监测工作的要求。

A high-performance liquid chromatography with electrochemical detection was developed for the determination of imipramine and its metabolites in plasma. The mobile phase consisted of 1% triethylamine (pH 3.0,adjusted with phosphoric acid)-methanol-tetrahydrofuran (60:30:10, V/V)and electrochemical detection at 0.7 V.The detection limit for imipramine, desipramine, 2-hydroxyimipramine and 2-hydroxydesipramine were 0.5, 0.5, 0.6 and 0.8 μg/L, respectively. The mean recovery were 86.9%, 88.4%, 82.5% and 78.8%, respectively.The coefficient of variation(CV%) of within-day were less than 3.96, 4.72, 6.05 and 6.64, respectively. The between-day CV% were less than 5.63, 5.33, 4.95 and 6.24, respectively. The method is simple and sensitive enough to be used for clinical pharmacological research and therapeutic drug monitoring.