绒癌细胞靶向微泡造影剂结合特性研究

Binding of targeted a microbubble contrast agent to choriocarcinoma cells in vitro

目的探索体外靶向SonoVue微泡与绒癌细胞的结合特性、结合率及靶向微泡的稳定性,为临床进行滋养细胞肿瘤的超声定位提供依据。方法用SonoVue微泡与兔抗人HCG抗体作用制成靶向造影剂,然后分别与绒癌细胞及子宫内膜间质细胞作用,比较各自的结合率及冲洗前后的结合率。结果SonoVue微泡与兔抗人HCG抗体的结合率为77.6%;绒癌细胞与靶向SonoVue微泡的花环形成率为(87.8±6.3)%,明显高于子宫内膜间质细胞花环形成率的(9.4±1.7)%(P<0.05);靶向SonoVue微泡与贴壁生长的绒癌细胞结合率为(85.4±4.7)%,冲洗后结合率为(83.1±3.8)%(P>0.05);流式细胞仪检测绒癌细胞与靶向SonoVue微泡的结合率为81.0%。结论在体外靶向SonoVue微泡与绒癌细胞可特异性结合,其结合力具有一定的强度,可望能抵抗毛细血管内血流生理性冲洗的破坏,实现靶向显影。

Objective To observe the cell binding characteristics of SonoVue microbubbles targeting choriocarcinoma cells and provide evidence for clinical ultrasonic localization of the tumor utilizing the microbubbles. Methods The targeted microbubbles were prepared by conjugating anti-HCG antibody with the SonoVue microbubbles and added in choriocarcinoma cells or endometrial stromal cells to compare the cell binding rate of the agents under optical microscope and with flow cytometry. Results Flow cytometry demonstrated a binding rate of 77.6% between the SonoVue microbubbles and anti-HCG antibody. Light microscopy showed that the total rosette formation rate of the choriocarcinoma cells exposed to the targeted microbubble beating anti-HCG antibody reached （87.8±6.3）%, significantly higher than that of the endometrial stromal cells [（9.4±1.7）%, P〈0.05 ]. The binding rate of the targeted microbubbles with the choriocarcinoma cells before and after PBS washing were （85.4±4.7）% and （83.1±3.8）% （P〉0.05）, respectively, suggesting strong stability of the binding. The binding rate was 81.0% according to the results offtow cytometry. Conclusion The targeted microbubbles as a contrast agent can efficiently bind to the choriocarcinoma cells in vitro with a stability sufficient to resist the blood flow.