结直肠癌组织中XIAP的表达及其意义

Expression of X-linked inhibitor of apoptosis protein in colorectal cancer tissues

目的探讨结直肠癌组织中凋亡抑制蛋白XIAP的表达及其与各临床病理因素的关系。方法应用免疫组织化学染色法以及RT-PCR检测87例结直肠癌组织中XIAP的表达情况。结果结直肠癌、癌旁组织、正常组织中XIAPmRNA表达阳性率分别为64.4%(56/87)、49.4%(43/87)和11.4%(10/87),结直肠癌组织中XIAPmRNA表达阳性率与癌旁组织和正常组织比较均有显著性差异。免疫组化染色显示,87例结直肠癌组织有61例(70.1%)XIAP呈阳性,XIAP表达在不同病理分级之间差异有显著性(P=0.014),在不同的临床分期、肿瘤部位、淋巴结转移之间的差异无显著性。结论XIAP在结直肠癌中的高表达与结直肠癌的发生可能有关,其是一种具有潜在价值的结直肠癌肿瘤标志物。

To investigate the expression of X-linked inhibitor ofapoptosis protein （XIAP） in colorectal cancer tissues and investigate its correlation with the clincopathological factors of the malignancy. Methods Immunohistochemistry and RT-PCR was employed to detect the expression of XIAP in 87 colorectal cancer tissues. Results XIAP mRNA expression was detected in 64.4% （56/87） of the colorectal cancer tissues, 49.4% （43/87） of the adjacent tissues, and in 11.4% （10/87） of the normal tissues, respectively. The cancer tissues showed significant greater positivity rate and higher expression level of XIAP mRNA than the adjacent and normal tissues. Immunohistochemistry also identified a significantly greater positivity rate for XIAP [70.1% （61/87）] in the colorectal cancer tissues. The pathological grade of the tumors was associated with the expression level of XIAP, whereas this association was not established between XIAP expression and the clinical stages, tumor position or lymph node metastasis. Conclusion XIAP may play an important role in the development of colorectal cancer, which might serve as a potentially useful tumor marker.