薤白对气滞型血管内皮损伤相关基因谱的影响

Effect of Bulbus Allii Macrostemi on Gene Expression Profile Associated with Vascular Endothelium Injure of Qi Stagnation Rats

目的:探讨薤白对气滞型血管内皮损伤相关的炎症、氧化应激等基因表达谱的影响。方法:用高L-蛋氨酸复制SD大鼠血管内皮损伤,并附以束缚法制造气滞证实验模型。应用RT-PCR技术和NCBI提供的SAGE(serialanalysis of gene expression)数据库及在线工具,分析内皮损伤相关的炎症、氧化应激基因表达谱的变化,以及薤白对基因表达谱的影响。结果:与模型组相比,薤白组炎症相关COX-2、COX-1,氧化应激相关iNOS及血管舒缩相关ECE、eNOS的基因表达降低(P<0.05或P<0.01),抗氧化SOD的基因表达增加(P<0.01)。结论:薤白能够改善血管病变时基因表达谱的异常,从而起到保护血管内皮免受损伤的作用。

Objective： To investigate the effect of Bulbus Allii Macrostemi on gene expression profile associated with inflammation and oxidative stress in vascular endothelium injure of qi stagnation rats. Methods： The model of vascular endothelium injure of qi stagnation rats were established by using high L-Methionine and being fastened. RT-PCR and serial analysis of gene expression （SAGE） database, available in NCBI, were used to analyze the changes of genes expression related to inflammation and oxidative stress in endothelium injure and the effect of Bulbus Allii Macrostemi. Results： Compared with model group, the genes expression of inflammation related COX-1 ,COX-2, oxidative stress related iNOS and eNOS,ECE involving in vascular vasomotion decreased （P 〈 0. 05 or P 〈 0. 01 ）, Hut the gene expression of antioxidative SOD increased （ P 〈0. 01 ） in Bulbus Allii Macrostemi group. Conclusion： Bulbus Allii Macrostemi can adjust the disorder of the genes expression, related to vascular endothelium injure in qi stagnation rats, protecting the vascular ehndothelium from injure.