周期型马来丝虫人工感染杂交小鼠的实验研究

Experimental Study on Hybridized Mice with Brngia malayi Artifical Infection

在实验室首次让长爪沙鼠和小白鼠进行杂交，得MKG杂交小鼠。从东乡伊蚊体内收集周期型马来丝虫感染或幼虫200～250条注入杂交鼠腹腔内，125～284d在12只杂交鼠腹腔抽液中4只检获微丝蚴，密度为5～353条／60μl，其形态大小与沙鼠和人体内的无可见差异。感染30～165d的16只杂交小鼠，解剖9只，有6只检获丝虫是虫或成虫。实验感染率为56.25％（9／16）。丝虫杂交鼠模型的建立，为丝虫生物学、病理学、免疫化学、新药筛选和分子生物学（DNA探针）等研究开辟新的途径。

We have employed Mongolian jirds(Meriones unguiculatus)and Kunming mice to hybridize a new MKG mouse.Infective larvae(L3)of periodic Brugia malayi were harvested from Aedes togoi mosquitoes and inoculated into peritoneal cavity of 16 MKG mice(200-250 L3 for each mouse). The primary appearance of microfilariae in 4 MKG mice were in 125～284 days after infection.The Mf density was 5～353/60μl in peritoneal fluid.The morphology was similar to those in jirds and human. In 9 MKG mice were necropsied and juvenile or adult worms were found in 6 MKG 30～165 days after infection. The infective positive rate was 56.25% (9/16). Our results demonstrate that Brugia malayi hybridized mouse animal model was first established in our laboratory and could be used on study filariasis biology,pathology,immunology,biochemistry,screening of antifilarial drugs and molecular biology.