摘要
本试验采用SDS-PAGE对10株嗜水气单胞菌(Ah)的外膜蛋白(OMP)和脂多糖(LPS)进行分析,结果显示大多数菌株具有相似的OMP图谱,而LPS则有较大的差异,用鲫鱼抗AhJ-1株全菌血清进行免疫转印,发现S蛋白(S-layerProtein)是70%Ah菌株的共同抗原,而LPS则有明显的抗原多样性,AhJ-1株抗血清仅能与同源菌株起反应。用AhJ-1株HEC毒素(Hemolvticactivity,Enterotoxicity,Cytotoxicity,HEC)的单克隆抗体检测此10株Ah,8株细菌可产生抗原性相同的HEC毒素。用纯化的AhJ-1株的S蛋白、LPS及HEC毒素(预先天活)分别免疫小鼠,强化免疫后,对同源菌株的攻击均能完全保护;S蛋白免疫组和HEC毒素免疫组对异源菌株TPS-30的攻击均能保护,而LPS免疫组则不能完全保护。上述结果表明,S蛋白和HEC毒素是Ah的主要共同保护抗原。AhJ-1株有望成为预防嗜水气单胞菌败血症的疫苗生产菌株。
Outer membrane protein(OMP) and lipopolysaccharide(LPS) of Aeromona hydrophila were analyzed by SDS -PAGE. The results showed that different strains of Aeromonas hydrophila had different LPS patterns but similar OMP profiles.Immunoblotting with antibodies against whose cell antigen of A. hydrophila strain J-1(Ah J-1)revealed that S-layer protein was a common surface antigen among A. hydrophila. Antigenicity of S-layer proteins of A. hydrophila(7/10) was the same as that of Ah J-1,but LPS presented divergent antigenicity.Most strains of A.hydrophila(8/10) produced HEC toxin with the same antigenicity, as indicated by DoT-ELISA with monoclonal antibodies against HEC toxin of Ah J-1. Not only HEC toxin but also S-layer protein and LPS of Ah J-1 induced strong protective immune response against challenge with homogeneous virulent bacterial strain.It could be concluded that S-layer protein and HEC toxin are common protective antigens of A. hydrophila and Ah J-1 may be used as vaccine-producing strain.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1997年第3期20-23,共4页
Chinese Journal of Zoonoses
关键词
嗜水气单胞菌
共同保护抗原
S蛋白
Aeromonas hydrophila
S-layer protein
HEC toxin
common protective antigen