日本血吸虫病患者血清CIC解离后特异性检测抗原的进一步研究

Further Studies on Specific Detection of Antigen in Circulating Immune Complexes(CIC) Dissociation in Sera from Schistosomiasis cases

将受检者血清免疫复合物（CIC）解离后，以抗-SEAPcAb作ELISA特异性检测非游离性循环抗原（NF－CAg）。通过30例不同病期患者血清、已治疗血吸虫病人血清、其它寄生虫病患者血清和健康人血清的对比检测结果表明，急性、慢性和晚期血吸虫病人的阳性率分别为76.7％、80.0％和60．0％，且三者的NF－CAg水乎（OD均值）均显著高于健康者（P均＜0．01），其它寄生虫病患者和健康人的交叉反应率和假阳性率分别为5．0～10.0％和8.8％，前者检测的NF－CAg水平，均接近健康入水平。经治疗后3个月、6个月及3年的血吸虫病入血清检测结果转阴率分别为86．7％、82．0％和96．7％，且其NF－CAg水平（OD值），均接近或低于健康人的水平。上述结果进一步表明，血吸虫病人血清CIC解离后特异性检测NF－CAg具有较好的诊断病人和考核疗效的价值。

The specific non-free circulating antigen(NF-CAg) of dissociated CIC in sera from 370 cases was detected by ELISA with anti-SEA polyclonal antibody. The cases consisted of acute,chronic and advanced schistosomiasis,cured schistosomiasis,other parasitosis and healthy individuals. The positive rates of acute,chronic and advanced schistosomiasis cases were 76.7%, 80.0% and 60.0% respectively. The NF-CAg level(mean OD values) of this group was higher than those of healthy individuals significantly(P＜0.01). The cross reaction rates with other parasitosis cases were 5.0～10.0% and the false positive rate of healthy individuals was 8.8%. The turned negative rates treated after 3 months, 6 months and 3 years were 86. 7 %,82. 0% and 96.7% respectively,and the NF-CAg levels of all those were approaching or lower than those healthy individuals. The results further showed that the specific NF-CAg of dissociated CIC was possessed of value for the diagnosis and the eveluation of effective chemotherapy.