摘要
将受检者血清免疫复合物(CIC)解离后,以抗-SEAPcAb作ELISA特异性检测非游离性循环抗原(NF-CAg)。通过30例不同病期患者血清、已治疗血吸虫病人血清、其它寄生虫病患者血清和健康人血清的对比检测结果表明,急性、慢性和晚期血吸虫病人的阳性率分别为76.7%、80.0%和60.0%,且三者的NF-CAg水乎(OD均值)均显著高于健康者(P均<0.01),其它寄生虫病患者和健康人的交叉反应率和假阳性率分别为5.0~10.0%和8.8%,前者检测的NF-CAg水平,均接近健康入水平。经治疗后3个月、6个月及3年的血吸虫病入血清检测结果转阴率分别为86.7%、82.0%和96.7%,且其NF-CAg水平(OD值),均接近或低于健康人的水平。上述结果进一步表明,血吸虫病人血清CIC解离后特异性检测NF-CAg具有较好的诊断病人和考核疗效的价值。
The specific non-free circulating antigen(NF-CAg) of dissociated CIC in sera from 370 cases was detected by ELISA with anti-SEA polyclonal antibody. The cases consisted of acute,chronic and advanced schistosomiasis,cured schistosomiasis,other parasitosis and healthy individuals. The positive rates of acute,chronic and advanced schistosomiasis cases were 76.7%, 80.0% and 60.0% respectively. The NF-CAg level(mean OD values) of this group was higher than those of healthy individuals significantly(P<0.01). The cross reaction rates with other parasitosis cases were 5.0~10.0% and the false positive rate of healthy individuals was 8.8%. The turned negative rates treated after 3 months, 6 months and 3 years were 86. 7 %,82. 0% and 96.7% respectively,and the NF-CAg levels of all those were approaching or lower than those healthy individuals. The results further showed that the specific NF-CAg of dissociated CIC was possessed of value for the diagnosis and the eveluation of effective chemotherapy.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1997年第3期38-40,共3页
Chinese Journal of Zoonoses
基金
国家8.5攻关课题
关键词
日本血吸虫病
解离
循环抗原
血清免疫复合物
Schistosomiasis,CIC dissociation,Non-free circulating antigen,The evaluation of effective chemotherapy