TNF-α、IL-6、IL-8在军团病中的检测及意义

DETECTION AND SIGNIFICANCE OF INTERLEUKIN-6、INTERLEUKIN-8、TUMOR NECROSIS FACTOR-α IN PATIENTS WITH LEGIONNAIRES DISEASE

[目的]本研究旨在探讨细胞因子IL-6、IL-8、TNF-α在军团病(LD)患者血清中的表达情况及其临床意义。[方法]用ELISA法检测30例LD患者和40例健康体检者血清中IL-6、IL-8、TNF-α的含量并进行对比。[结果]LD患者血清中TNF-α、IL-6、IL-8均表现出急性期﹥恢复期,且急性期TNF-α、IL-8浓度均较正常对照组明显增高,P﹤0.05;IL-6与IL-8、TNF-α、及TNF-α与IL-8的改变存在明显的正相关。[结论]LD患者血清中IL-6、IL-8、TNF-α含量出现表达异常,并且随着病情程度的不同改变不同,对了解LP感染的发病过程、机体的免疫功能、病变损伤程度及预后的判断有重要的意义。

[Objective]To investigate the changes and significance of serum interleukin-6（IL-6）、interleukin-8（IL-8）and tumor necrosis factor-α（TNF-α）in patients with legionnaires disease（LD）.[Methods]The levels of IL-6、IL-8 and TNF-α in sera of 30 patients with legionnaires disease and 40 controls were detected with ELISA.[Results]The levels of serum IL-6、IL-8 and TNF-α of LD patients in acute stage were all higher than those in convalescent period.And the levels of serum IL-8、TNF-α of acute patients were significantly higher than those of the controls.The level of IL-6 had obvious positive correlation with the levels of serum IL-8 and TNF-α of LD patients.[Conclusions]The expressions of IL-6、IL-8 and TNF-α in serum of LD patients is abnormal and their serum levels changes along with the degree of pathogenetic condition.It is of important significance in understanding the course of LP infection invasion,the immunologic function of patients,the degree of pathological changes and injuries,as well as the prognosis.