薄层色谱法跟踪N-萘乙酰基-N′-邻甲氧基苯甲酰氨基硫脲的合成

TLC Tracking the Synthesis of N-Naphthylacetyl N′-o-Methoxy Benzoyl Thiosemicarbazone

通过α-萘乙酸和SOCl2在无水苯中反应制得α-萘乙酰氯,然后与硫氰酸钾反应生成α-萘乙酰基异硫氰酸酯,再与邻甲氧基苯甲酰肼进行加成反应,生成新型N,N′-萘乙酰基取代邻甲氧基苯甲酰氨基硫脲化合物,用薄层色谱法跟踪最后一步反应,确定了反应时间。产物经1H NMR、IR和元素分析测定。生物活性试验结果表明对稻子根生长具有促进作用,对大肠杆菌和枯草杆菌无明显抑菌作用。

α-Naphthylacetyl chloride is prepared in anhydrous benzene from α-naphthylacetic acid and SOCl2.The compound obtained then reacted with potassium thiocyanate to gave α-naphthylacetyl isothiocyanate.At last,a new type of N,N′-naphthylacetyl-o-methoxy benzoyl thiourea compound is obtained through the addition reaction of α-naphthylacetyl isothiocyanate and o-methoxy benzoyl hydrazine.The final step of the reaction is detected by TLC and the reaction time is determined.The product is confirmed by 1H NMR,IR and elemental analysis.The results of the biological activity tests show that the product could promote rice root growth and has no significant inhibitory effect to escherichia coli and bacillus subtilis.