鸡球虫三价弱毒疫苗对鸡的免疫效果研究

Immune efficacy of the 3-valent attenuated vaccine against coccidiosis in chickens

将100只3日龄罗曼雏鸡随机分成空白对照组、攻虫对照组、Ⅰ组(1个免疫剂量)、Ⅱ组(2个免疫剂量)和Ⅲ组(0.5个免疫剂量)共5个试验组,每组20只鸡,对研制的鸡球虫三价弱毒疫苗的安全性和免疫效果进行初步评价。结果,各组的相对增重率依次为100.0%、86.8%、81.8%、89.2%和89.7%;攻虫后第10 d剖检所有鸡,各组肠道病变记分依次为0、2.7、2.2、2.3和2.6;免疫期间,Ⅰ、Ⅱ、Ⅲ组的OPG值依次为5.7×106、6.7×106和7.3×106;攻虫期间,攻虫对照组的OPG值为12.3×106,Ⅰ、Ⅱ、Ⅲ组的OPG值依次为7.2×106、8.5×106和9.0×106。攻虫后各组的死亡率依次为0、5%、0、10%和0;各组的抗球虫指数依次为200.0、115.8、135.8、140.2和143.7。结果表明,该三价弱毒疫苗抗柔嫩艾美球虫的效果并不理想,还需要进一步改进和完善。

To examine the safety and immune efficacy of the 3-valent attenuated vaccine against chicken coccidiosis in chickens, one hundred 3-day-old Roman broilers were randomly divided into blank control group,challenge control group,Group Ⅰ （1 immune dose） ,Group Ⅱ （2 immune doses） and GroupⅢ （0. 5 immune dose） with 20 each. The average relative ratios of weight gain in the 5 groups were 100.0% ,86.8%, 81.8 %, 89.2 % and 89.7 %, respectively, and the intestinal lesion scores in the 5 groups was 0,2. 7,2. 2,2. 3 and 2.6,respectively. The oocysts per gram of feces（OPG） in the challenge control group was 12.3 ×10^6 , while the OPG values in the 3 immunized groups were 5.7 ×10^6 , 7 ×10^6 and 7.3 ×10^6 , respectively. After challenge,the OPG values of the challenge control group and the 3 immunized groups were 12.3 ×10^6 , 7.2×10^6 ,8.5×10^6 and 9.0×10^6 ,respectively. The mortalities in the 5 groups post-challenge were 0,5%, 0,10 % and 0, while ACIs were 200. 0,115. 8,135. 8,140. 2 and 143.7, respectively. These results revealed that the immune efficacy of the 3-valent attenuated vaccine against chicken coccidiosis was poor, so the vaccine should be further improved.