磁流体热疗对荷Lewis肺癌小鼠肿瘤细胞凋亡和周期的影响

Effect of Magnetic Fluid Hyperthermia on Apoptosis and Cell Cycle of Tumor Cells in Mice with Lewis Lung Carcinoma

目的探讨磁流体热疗对荷Lewis肺癌小鼠肿瘤细胞的凋亡和周期的影响。方法接种Lewis肺癌细胞悬液于C57BL/6小鼠的皮下,等肿瘤长至直径约为(0.8±0.1)cm时,随机分为4组:对照组、磁场组、磁流体组、实验组。加温治疗后48h,眼球取血,检测血中白细胞的变化,切取肿瘤标本,流式细胞仪检测细胞凋亡率和周期的变化。结果热疗后4组血中白细胞没有明显的变化(F=0.62,P=0.613);肿瘤细胞凋亡率实验组为(63.55±8.39)%,对照组、磁场组、磁流体组分别为(28.43±6.29)%,(32.75±5.07)%,(32.42±6.15)%,实验组肿瘤细胞凋亡率明显高于其他3组(q=11.925,P<0.05;q=10.458,P<0.05;q=10.570,P<0.05);实验组细胞周期出现明显G1/G0期阻滞为(68.13±5.73)%显著高于对照组(47.95±9.98)%(q=5.501,P<0.05),磁场组(49.23±6.62)%(q=5.152,P<0.05),磁流体组(52.28±9.64)%(q=4.320,P<0.05)。结论磁流体热疗可明显提高Lewis肺癌细胞的凋亡率,抑制Lewis肺癌细胞G1期向S期的进程。

Objective To study the effect of magnetic fluid hyperthermia on apoptosis and cell cycle of tumor cells in mice with Lewis lung carcinoma. Methods A total of 20 C57BL/6 mice were subcutaneously inoculated with Lewis lung carcinoma cells. When the tumor grew to （0.8 ±0.1 ） cm in diameter, the mice were randomly divided into four groups： control, magnetic, magnetic fluid, and experiment groups. Forty-eight hours after being treated with hyperthermia, blood samples were collected from the eyes of the mice, and the WBC was counted. The tumors were removed to evaluate the apoptosis rate and the cell cycles by flow eytometry. Results No significant change of WBC count was detected in the four groups after hyperthermia （ F = 0.62, P = 0. 613 ）. The apoptosis rate of the tumor cells in the experiment group was （63.55 ± 8.39） % , which was significantly higher than that in the control [（28.43 ±6.29）% ; q=11.925, P 〈0.05], magnetic [（32.75 ±5.07）%, q =10.458, P 〈0.053, and magnetic fluid groups [（32.42 ±6. 15）%; q =10.570, P 〈0.05]. In the experiment group, （68. 13 ±5.73）% of the tumor cells were arrested at GO/G1. The percentage was significantly higher than that in the other 3 groups [ （47.95 ± 9.98）% in the control, q = 5. 501, P 〈0.05; （49.23 ± 6.62）% in the magnetic group, q = 5. 152, P 〈 0.05; （52. 28 ± 9. 64）% in the magnetic fluid group, q = 4. 320, P 〈 0. 05）. Conclusions Magnetic fluid hyperthermia can increase the apoptotic rate of Lewis lung carcinoma cells, and inhibit the cell progress from G1 to S stage.