陕北白绒山羊H-FABP基因SNPs及其与生长、胴体性状的相关研究

Study on SNPs of H-FABP Gene and Its Relationship with Growth and Carcass Traits in Shanbei White Cashmere Goats

利用PCR-SSCP技术分析了262头周岁陕北白绒山羊H-FABP基因外显子2的多态性及其与生长和胴体性状的关系,结果表明:该座位存在1个突变位点(G→C),且该群体处于Hardy-Weinberg非平衡状态;H-FABP基因的AB与AA和BB基因型个体背膘厚差异显著,AA和BB型个体间的差异极显著;AB和BB型个体的胸围和管围差异显著(P<0.05),AA和BB型个体的眼肌面积具有显著差异(P<0.05),其他性状在不同基因型间均无显著差异;此外,在初生重、眼肌面积、背膘厚等6项指标上,均表现为AA>AB>BB,而在体高、胸围、管围3项指标上,均表现为AB>AA>BB。可以推断H-FABP基因可作为陕北白绒山羊生长及胴体性状的候选基因和绒山羊肉用性能的分子标记。

PCR-SSCP technique was used to analyze the polymorphisms at the exon 2 of the H- FABP gene in 262 yearling Shanbei White Cashmere goats to further investigate the correlation between polymorphism and growth and carcass traits. The results showed that there was one mutation （G to C） at this gene locus with the gene frequency of the population in Hardy-Weinberg disequilibrium. The backfat thickness of AB genotype of H-PFBP gene was significantly different from that of AA and BB （P 〈 0.05）, significant difference was found between the genotypes of AA and BB for the backfat thickness （P 〈 0.01） ; there was a significant difference between the genotypes of AB and BB for the heart girth and circumference of cannon （P 〈 0.05） as well as between the genotypes of AA and BB for the loin eye area, the significant differences between genotypes were not noted for other traits. Moreover, the values of birth weight, loin eye area, backfat thickness and other 3 traits ranked as AA〉AB〉BB, the values of body height, heart girth and circumference of cannon ranked as AB〉AA〉BB, which indicated that H-FABP gene may be a candidate gene for the selection of growth and carcass traits in Shanbei White Cashmere goats and a possible molecular marker for meat performance of cashmere goat.