奶牛临床型乳房炎与健康乳腺组织蛋白表达图谱的初步分析

Proteome Maps of Mammary Tissues in Healthy Dairy Cows and Those with Clinical Mastitis

本研究以二维电泳技术构建了临床型乳房炎奶牛和健康奶牛的乳腺组织全蛋白表达谱,运用PDQuest7.4软件进行差异分析。结果显示:健康奶牛的乳腺组织蛋白表达图谱可检测到(480±27)个蛋白点,临床型乳房炎奶牛可检测到(453±31)个蛋白点,二者存在17个差异表达的蛋白斑点,6个蛋白斑点在临床型乳房炎奶牛组织中呈量度,4个呈质变。表明临床型乳房炎奶牛和健康奶牛的乳腺组织蛋白存在差异表达的蛋白斑点,本研究为发现新的乳房炎调控分子和潜在的医疗目标蛋白奠定了基础。

In the mammary glands suffered from invading pathogens that produced and released inflammatory mediators might be accompanied by changes in protein expression profiles. This project presents the protein changes of the mammary gland from healthy dairy cows and clinical mastitic cows that were investigated using two-dimensional gel electrophoresis （2-DE）. Acquired gel maps were matched and analyzed using PDQuest 7.4 image analysis software. The results showed that the protein profiles in healthy dairy cows and those with clinical mastitis were shown to be very similar, 480±27 and 453±31 protein spots were statistically detected, respectively. Take ＂ more or less＂ as the determination criterion, 17 protein spots were detected to be differentially expressed changes in the stain density of threefold or more. That is, 10 protein spots were upor down-regulated proteins in clinical mastitic cows, 7 protein spots were unique in healthy dairy cows or clinical mastitic cows. The results indicated significant difference in expression protein between healthy dairy cows and those with clinical mastitis, and may provide valuable information to find new regulation markers of mastitis and potential protein targets for treatment.