三七皂苷对持续炎症加速兔动脉粥样硬化形成中氧化应激影响的研究

Effects of Total Saponins of Panax Notoginseng on Oxidative Stress During Atherosclerogenesis in Rabbits Accelerated by Persistent Inflammation

目的:初步探讨三七皂苷(PNS)对持续炎症加速兔动脉粥样硬化(AS)形成中氧化应激的影响。方法:将24只日本大耳兔随机均分为对照组、高脂组、炎症组和PNS组,各组给予相应治疗。动态监测各组动物血清中C反应蛋白(CRP)、一氧化氮(NO)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;测定胸主动脉AS斑块面积。结果:炎症组血清CRP、MDA含量显著增高,SOD活性和NO含量显著下降,胸主动脉AS斑块面积显著增加,且CRP、MDA含量与斑块面积均呈显著性正相关,与高脂组有显著性差异(P<0.01)。与炎症组比较,PNS组可减轻炎症AS症状,防止AS形成。结论:持续炎症增强氧化应激可能是加速高脂饮食兔AS的形成机制之一。PNS可降低氧化应激,减轻炎症反应,防治AS。

OBJECTIVE： To study the effects of Total sasponins of Pannax Notoginseng （PSN） on oxidative stress during atherosclerogenesis （AS） in rabbits accelerated by persistent inflammation. METHODS： A total of 24 Japanese big-eared rabbits were divided into control group, high lipid group, persistent inflammatory group, and PSN group to be treated ac- cordingly. Serum levels of C reactive protein （CRP）, nitric oxide （NO） and malondialdehyde （MDA） as well as the activity of superoxide dismutase （SOD） were given a dynamic monitoring; and aortic atherosclerotic plaque areas were measured. RE- SULTS： In the persistent inflammatory group, serum levels of CRP and MDA were increased significantly, but SOD activity and level of NO were decreased significantly, aortic atherosclerotic plaque area was increased obviously, and CRP level was positively correlated with both MDA content and plaque area, showing significant difference as compared with the high- lipid group（P 〈 0.01） .Contrary to the persistent inflammatory group, AS plaque areas in PNS group were diminished. CONCLU- SION： The oxidative stress reaction induced by persistent inflammation may contribute to atherosclerosis development in high lipid- fed rabbits. PNS can lessen the oxidative stress reaction and the inflammatory reaction so as to prevent and cure AS.