桑白皮药材中1-脱氧野尻霉素的两种含量测定方法的比较

Comparison of two determination methods of 1-deoxynojirimycin in Cortex Mori

目的:建立和比较两种测定桑白皮中1-脱氧野尻霉素(1-Deoxynojirimycin,简称DNJ)的方法。方法:方法1:采用Hypersil NH2柱,以乙腈-水(78∶22)为流动相;ELSD检测器。方法2:采用ZORBAX SB-C18柱,以乙腈-0.1%醋酸溶液(25∶75)为流动相;DAD检测器,检测波长为265nm。结果:两种HPLC法测定结果基本一致,经t检验无显著性差异(P>0.05)。结论:两种方法的重现性好,灵敏度高,均可有效控制桑白皮中DNJ的含量。

AIM： To develop and compare two HPLC methods for determination of 1-deoxynojirimycin （DNJ） in Cortex Moil. METHODS： method 1 ： This method used a Hypersil NH2 column and with a mobile phase consisted of acetonitrile-water（78： 22） ; Detector was ELSD. method 2： This method used a ZORBAX SB-C18 analysis col- umn and with a mobile phase consisted of acetonitrile-0. 1% acetic acid solution （25：22 ） ; DAD Detector wave- length was at 265 nm. RESULTS： Two methods had almost the same result and not remarkable difference by t test （P 〉 0.05）. CONCLUSION： Two methods are of good reproducibility and sensitivity, and can be used for the quality control of DNJ in Cortex Mori.