美国岩榆愈伤组织的诱导及不定芽形成

Callus Induction and Bud Formation of Ulmus thomasii

以顶芽、嫩茎、叶片为外植体,对美国岩榆进行组织培养的初步研究。结果表明,用顶芽作外植体诱导愈伤组织的最佳培养基为MS+BA2.0mg·L-1+IBA2.0mg·L-1,诱导愈伤增殖的最佳培养基为MS+BA1.0mg·L-1+NAA1.0mg·L-1+IBA1.0mg·L-1;以幼叶为外植体产生愈伤的最佳培养基为MS+BA2.0mg·L-1+IBA2.0mg·L-1,诱导其愈伤增殖的最佳培养基为MS+BA1.0mg·L-1;用嫩茎段作外植体诱导愈伤组织的最佳培养基配比为MS+BA2.0mg·L-1+NAA0.5mg·L-1。诱导不定芽分化产生的外植体是嫩茎段,其最佳培养基组合为MS+BA1.0mg·L-1+NAA1.0mg·L-1+IBA1.0mg·L-1。

The callus of Ulmus thomasii was induced to form by using the tip bud, young stem and young leaf as explants. Result shows that the optimum culture medium for callus induction with tip bud as explants is MS ＋ BA 2.0mg·L^-1 ＋ IBA 2.0mg·L^-1 , that for callus proliferation is MS ＋ BA 1.0mg·L^-1 ＋ NAA 1.0mg·L^-1 ＋ IBA 1.0mg·L^-1. As for leaves, the optimal culture medium for callus induction is MS ＋ BA 2.0mg·L^-1 ＋ IBA 2.0mg·L^-1, and the culture medium, MS ＋ BA 1.0mg·L^-1 , is suitable for callus proliferation. The appropriate culture medium for callus induction with young stem as explants is MS ＋ BA 2.0mg·L^-1 ＋ NAA 0.5mg·L^-1. The callus from leaf and tip bud could not induce to form adventitious buds, while the callus from young stem could induce to form adventitious buds using MS ＋ BA 1.0mg·L^-1 ＋ NAA 1.0mg·L^-1 ＋IBA 1.0mg·L^-1 as culture medium.