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Klebsiella oxytoca HP1乙醇脱氢酶基因敲除对产氢的影响(英文) 被引量:1

Effects of adhE Gene Knock-out on Hydrogen Evolution of Klebsiella oxytoca HP1
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摘要 Klebsiella oxytoca HP1是从温泉筛选得到的很有潜力的产氢菌株。为了进一步提高产氢能力,应用同源重组技术构建了乙醇途径缺少菌株,并分析和比较了野生菌和工程菌的代谢流量分布。 Klebsiella oxytoca HP1 is a potential H2-producing bacterium we screened from a hot spring. In the present study, metabolic engineering strategy was carried out to construct ethanol pathway mutants by homologous recombination, in order to improve hydrogen production yield. Metabolic fluxes of the wild and the mutants were analysed and compared.
出处 《中山大学学报(自然科学版)》 CAS CSCD 北大核心 2007年第B06期327-328,共2页 Acta Scientiarum Naturalium Universitatis Sunyatseni
基金 国家自然科学基金资助项目(30470395)
关键词 代谢工程 发酵产氢 KLEBSIELLA oxytoca HP1 adhE基因 metabolic engineering hydrogen production Klebsiella oxytoca HP1 adhE
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参考文献4

  • 1LONG M N, HUANG J L, WU X B. Isolation and characterization of a high H2-producing strain Klebsiella oxytoca HP1 from a hot spring. Research in Microbiology [J]. 2004, 156 (1): 76-81.
  • 2DAS D, VEZIROGLUB T N. Hydrogen production by biological processes : A survey of literature [ J ]. Int J Hydrogen Energ, 2001, 26:13-28.
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同被引文献24

  • 1Show KY, Lee D J, Tay JH, et al. Biohydrogen production: Current perspectives and the way forward. Int J Hydrogen Energy, 2012, 37(20): 15616-15631.
  • 2Fan Z, Yuan L, Chatterjee R. Increased hydrogen production by genetic engineering of Escherichia coli. PLoS ONE, 2009, 4(2): e4432.
  • 3Heuser F, Schroer K, Liitz S, et al. Enhancement of the NAD(P)(H) pool in Escherichia coli for biotransformation. Eng Life Sci, 2007, 7(4): 343-353.
  • 4Ying W. NAD+ and NADH in cellular functions and cell death. Front Biosci, 2006, 11(9): 3129-3148.
  • 5Zhang C, Lv FX, Xing XH. Bioengineering of the Enterobacter aerogenes strain for biohydrogen production. Bioresour Technol, 2011, 102(18): 8344-8349.
  • 6Kumar N, Das D. Enhancement of hydrogen production by Enterobacter cloacae IIT-BT 08. Process Biochem, 2000, 35(6): 589-593.
  • 7Tanisho S, Ishiwata Y. Continuous hydrogen production from molasses by the bacterium Enterobacter aerogenes. Int J Hydrogen Energy, 1994, 19(10): 807-812.
  • 8Bai LP, Wua XB, Jiang L J, et al. Hydrogen production by over-expression of hydrogenase subunit in oxygen-tolerant Klebsiella oxytoca HP 1. Int J Hydrogen Energy, 2012, 37(17): 13227-13233.
  • 9Berrios-Rivera S J, San KY, Bennett GN. The effect of NAPRTase overexpression on the total levels of NAD, the NADH/NAD~ ratio, and the distribution of metabolites in Escherichia coli. Metab Eng, 2002, 4(3): 238-247.
  • 10Self WT, Hasona A, Shanmugam KT. Expression and regulation of a silent operon, hyf, coding for hydrogenase 4 isoenzyme in Escherichia coli. J Bacteriol, 2004, 186(2): 580-587.

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