抗生素联用对D-检验阳性葡萄球菌体外抗菌活性的分析

Analysis of in vitro antibacterial activity of combined usage of antimicrobial agents against D-test positive Staphylococcus

目的了解医院内感染抗生素联合应用对D-检验(D-test)阳性葡萄球菌的体外抗菌活性。方法D-test检测方法和最小抑菌浓度琼脂平板稀释法按临床实验室标准协会规定的标准进行。结果39株D-test阳性葡萄球菌对万古霉素与头孢唑啉、左氧氟沙星、阿米卡星和左氧氟沙星与阿米卡星、头孢唑啉联合用药的最小抑菌浓度分别为1.0、1.0、1.0、2.0、4.0mg/L。结论对葡萄球菌引起的感染,细菌室应作D-test检测,为临床及时准确地提供药敏试验结果和病原菌的耐药表型检测结果。临床应根据患者感染的个体情况和药敏试验结果,结合经验用药的临床疗效正确选择抗生素。

Objective To understand in vitro antibacterial activity of combined usage of antimicrobial agents against D-test positive staphylococcus in nosocomial infection. Methods D-test detection method and agar plate dilution method for MIC were performed according to the rated standard of Clinical Laboratory Standard Institute （CLSI）. Results The minimal inhibitory concentration （MIC） 90 of 39 strains of D-test positive Staphylococci was 1.0, 1.0, 1.0, 2.0 and 4. 0 mg/L to vancomycin （VAN） combined with cefazolin （CFZ）, levofloxacin （LVF） alone, amikacin （AMK） alone, LVF combined with AMK, as well as LVF combined with CFZ. Conclusion D-test ought to be carried out by the clinical bacterial laboratory for Staphylococcal infected samples, so as to provide prompt and accurate susceptibility test result and pathogen drug resistance phenotype for clinic. Antimicrobial agents should be chosed rationally according to the individual infected statue, susceptibility test result, as well as clinical therapeutic effect of experiential therapy.