血清瘦素、总胆汁酸联合检测对妊娠肝内胆汁淤积症患者的诊断价值

Diagnostic value of combined determination of serum leptin,TBA level in patients with intrahepatic cholestasis of pregnancy

目的探讨妊娠肝内胆汁淤积症(ICP)患者血清瘦素(leptin)、总胆汁酸(total bile acids,TBA)水平对ICP患者母体并发症及围生儿的关系。方法检测91例ICP产妇(其中轻度42例、中度38例、重度91例)和34例健康晚期妊娠妇女(对照组)leptin和TBA水平,TBA测定采用TBA试剂盒,第5代循环酶法,leptin测定采用酶联免疫测定法。结果ICP产妇leptin和TBA水平明显高于对照组,差异有统计学意义(P<0.01),ICP患者母体并发症及围生儿患病率也明显高于对照组,差异有统计学意义(P<0.05),重度组患者与中度、轻度组比较差异有统计学意义(P<0.05)。结论leptin和TBA联合检测对妊娠ICP患者早期诊断和治疗、母体并发症及围生儿病情观察及预后有重要意义。

Objective To investigate the relationship between the level of serum leptin, total bile acid （TBA） and the maternal complications as well as perinatals during intrahepatic cholestasis of pregnancy （ICP）. Methods 91 lying-in women with ICP and 34 normal late pregnant women were enrolled in study. The 91 patients with ICP were divided into mild （n=42）, moderate （n=38） and serious group （n=91）. Serum leptin was assayed by total bile acid detection kit （5 cycle enzymatic method）, and TBA by enzyme-linked immunosorbent assay （ELISA）. Results The levels of serum leptin and TBA, maternal complications and perinatal morbidity were all remarkably higher in ICP group than those in control group. The differences were statistically significant. The above indice were signicantly higher in severe group than those in mild group and moderate group （P〈0.05）. Conclusion Combined determination of serum leptin and TBA may contribute to early diagnosis and treatment of ICP, pathogenetic condition viewing and prognosis evaluation of maternal complications and perinatals.