摘要
本文主要研究了弱碱高温处理法去除花生粕中黄曲霉毒素的效果,并对去毒后花生粕的酶解效果进行了研究。研究结果表明:弱碱高温处理对原料中的黄曲霉毒素有较好的去除效果并有利于酶解的进行,花生粕经过121℃、pH10、60min处理,黄曲霉毒素的破坏率高达84.50%,最终酶解上清液中黄曲霉毒素的含量低至0.344ng/mL;而此时酶解液中蛋白质回收率比对照组的提高率为27.44%,氨态氮含量比对照组的提高率为57.70%。
The effects of alkali and high temperature treatments on the aflatoxins degradation and enzymatic hydrolysis of peanut dregs are studied in this paper. Results show that the alkali and high temperature treatments contribute to the degradation of aflatoxins in peanut dregs and the enzymatic hydrolysis of peanut dregs. The optimized conditions are as follows： temperature of 121 ℃, pH value of 10 and treatment time of 60 min, under which 84.50% ofaflatoxins are decomposed and the concentration of the aflatoxins in the enzymatic hydrolysate is 0.344 ng/ml. Besides, the recovery rate of protein and amino nitrogen content in the hydrolysate are 27.44% and 57.70% respectively higher than those of the control group.
出处
《现代食品科技》
EI
CAS
2007年第11期4-6,共3页
Modern Food Science and Technology
基金
国家863计划专题(植物蛋白酶法改性共性关键技术研究2006AA10Z326)
广东省科技计划项目(粤港关键领域重点突破招标项目200649861112)
关键词
花生粕
黄曲霉毒素
酶解
酶联免疫
peanut dregs
aflatoxin
enzymatic hydrolysis
enzyme linked immunosorbent assay（ELISA）
