介孔材料SBA-15固定化胰蛋白酶的研究

Immobilization of Trypsin on Mesoporous Silica Material

在酸性条件下以正硅酸乙酯(TEOS)为硅源、非离子表面活性剂Pluronic P123为模板剂合成了介孔分子筛SBA-15,将其作为载体,对胰蛋白酶进行了固定化研究。研究了固定化条件对固定化酶量的影响,以及固定化酶的热稳定性和操作稳定性。结果表明,当酶液浓度大于5mg/mL,固定化时间10h时,胰蛋白酶的固定化效果最好,固定化酶量可达23.6mg/g。固定化酶的热稳定性与游离酶相比有了显著的提高,且固定化酶具有较好的操作稳定性,连续反应6批次后,酶的剩余活性仍保持在40%以上。

The hexagonal silica mesoporous material SBA-1 5 with ordered 2-dimensional channel structure was used as a support to immobilize the enzyme trypsin which was assembled in the channel of SBA-15 by immersion method. The effects of immobilization conditions such as enzyme concentration, pH value and time on the absorbed amount of enzyme in SBA-15, the thermal and operational stabilities of the immobilized enzyme were studied. And the best enzyme concentration and immobilization time were 5 mg/ml and 10 h, respectively, under which the absorbed amount of enzyme reached 23.6 mg/g. It was also found that the thermal and operational stabilities of the immobilized enzyme were higher than those of the free enzyme. Silica mesoporous material SBA-5 was shown to be a good support for immobilization of enzyme.