高糖对糖尿病患者内皮祖细胞增殖凋亡的影响及其机制探讨

Effects of high-glucose on proliferation and apoptosis in endothelial progenitor cells of type 2 diabetes mellitus

目的:了解葡萄糖对糖尿病患者内皮祖细胞(EPCs)增殖及凋亡的影响,并探讨其可能机制。方法:以不同浓度葡萄糖分别作用于25例正常人(对照组)和25例2型糖尿病患者(糖尿病组)EPCs,MTT法检测其增殖,Annexin-V/PI法检测其凋亡。RT-PCR法检测bcl-2和bax的表达水平。结果:33mmol/L葡萄糖使糖尿病组和对照组EPCs增殖率均减低,凋亡率增高;且能使糖尿病组和对照组EPCs表达bax增强,bcl-2表达无明显改变。而5mmol/L葡萄糖作用下糖尿病组和对照组EPCs增殖率、凋亡率及bax和bcl-2表达无明显改变。结论:高糖使EPCs增殖功能减弱,凋亡增加,bax表达增加可能是其机制之一。

AIM ： This study aimed to observe the effects of high - glucose on proliferation and apoptosis of endothelial progenitor cells （EPCs） in type 2 diabetes mellitus patients, and tried to elucidate their possible role. METH- ODS： Various concentrations of glucose were added to the culture system of EPCs from 25 cases of type 2 diabetes mellitus patients （DM group） and 25 cases of healthy volunteers （control group）. MTT assays were used to detect the proliferative rates. Annexin - V/PI stains were used to detect the apoptotic rates, and RT - PCR to detect the expression level of bcl - 2 and bax. RESULTS ： Proliferative activity of EPCs in both control group and DM group were attenuated when concentration of glucose was 33 mmol/L, while apoptotic rates increased. No significant change of proliferative rate and apoptotic rate of EPCs in DM group and control group in the presence of 5 mmol/L glucose was observed. The expression level of bax of EPCs in both DM group and control group increased while expression level of bcl -2 did not change much in the presence of 33 mmol/L glucose. CONCLUSION ： High - glucose attenuates proliferative activity of EPCs and increases the apoptotic rate. Upregulation of bax may be its possible role.