摘要
目的:研究我国胃癌高发区IL-1B-511基因多态性、幽门螺杆菌(Hp)感染与胃黏膜萎缩的关系。方法:研究对象为胃癌高发区(陕西)健康志愿者500例,人群按照年龄分成20-29岁、30-39岁、40-49岁、50-59岁、60岁以上等5组,每组100例。IL-1B-511基因多态性的分析采用限制性片段长度多态性(PCR-RFLP)方法,以血清胃蛋白酶原Ⅰ(PGⅠ)浓度和PGⅠ/PGⅡ比值作为胃黏膜萎缩的指标,血清PGⅠ、PGⅡ浓度和抗Hp-IgG抗体的检测均采用酶联免疫吸附试验(ELISA)检测。结果:随着年龄的增长,血清PGⅠ的浓度与PGⅠ/PGⅡ的比值呈逐渐下降的趋势;在各年龄组中,IL-1B-511T/T基因型Hp阳性者其血清PGⅠ及PGⅠ/PGⅡ低于相应年龄组Hp阴性者(P<0.05);多因素回归分析提示"IL-1B-511T/T基因型"、"年龄段"和"Hp"对"PGⅠ/PGⅡ比值"有显著影响(P<0.05、P<0.05和P<0.01)。结论:我国胃癌高发区胃黏膜萎缩的发生与IL-1B-511T/T基因型、年龄和Hp感染有密切关系,IL-1B-511T/T基因型增加Hp感染后胃黏膜萎缩发生的危险性。
AIM： To study the relationship between IL-1B-511 single nucleotide polymorphism, H. pylori infection, and gastric atrophy in high prevalent （Shanxi） region in China. METHODS： Five hundred healthy volunteers from Shanxi Province of China were recruited in this study, which were divided into five subgroups according to age, namely age 20 - 29, 30 - 39, 40 - 49, 50 - 59 and 〉 60 years, respectively. Genomic DNA was extracted from peripheral blood. IL-1B-511 single nucleotide polymorphism was analyzed by PCR-RFLP. Serum pepsinogen was used as a bio- marker of gastric atrophy. Serum pepsinogen I （ PG I ）, pepsinogen Ⅱ （ PG Ⅱ） and anti-H. pylori IgG were deter-mined by an ELISA assay. RESULTS： The mean serum PGI concentration and ratio of PG I /PGⅡ decreased gradually with increasing age, and were lower in subjects with IL-1B-511 T/T genotype and H. pylori infection than those without H. pylori infection in each subgroup, respectively （ All P 〈 0. 05 ）. Multiple linear regression showed that IL-1B-511 T/ T genotype, age and lip infection were significantly associated with gastric atrophy （ P 〈 0. 05, P 〈 0. 05 and P 〈 0. 01, re-spectively）. CONCLUSION： Gastric atrophy is closely correlated with IL-1B-511 T/T genotype, age, H. pylori infection in high prevalence region of gastric cancer in China. The＇ risk of gastric atrophy is significantly increased for the IL-1B-511 T/T genotype with lip infection.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2007年第11期2226-2228,共3页
Chinese Journal of Pathophysiology
基金
广东省医学科研基金资助项目(NoA2002176)
