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SGK1在糖尿病小鼠肾脏皮质中时间差异性表达及意义 被引量：1

Temporal differential expression of SGK1 gene in renal cortex of diabetic mice
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摘要 目的:研究血清和糖皮质激素诱导的蛋白激酶1(serum and glucocorticoid-inducible kinase1,SGK1)在糖尿病(DM)小鼠肾脏皮质中时间上差异性表达及意义。方法:采用链脲佐菌素(STZ)单次腹腔注射诱导小鼠糖尿病模型。将2月龄C57BL/6小鼠随机分成10组:第1、2、3、4、6、8、12周糖尿病小鼠组(DM1、DM2、DM3、DM4、DM6、DM8、DM12)和相应的第1、4、8周正常对照组(N1、N4、N8)。分别在DM模型制作成功后第1、2、3、4、6、8、12周末收集小鼠24h尿量,检测24h尿蛋白量;观察肾脏病理改变;半定量RT-PCR检测小鼠肾脏皮质SGK1及结缔组织生长因子(CTGF)mRNA的表达,Western blotting检测N4、DM4、DM8组皮质SGK1、CTGF蛋白的表达。结果:随着糖尿病病程的进展,DM组小鼠肾小球体积逐渐增大、系膜区逐渐增宽;DM组小鼠24h尿蛋白从第2周起开始逐渐增多,肾脏皮质SGK1mRNA表达从第2周开始明显上调,在第4周达到峰值。CTGF随着糖尿病病程的进展表达逐渐递增。结论:SGK1在糖尿病早期肾脏表达峰值的出现及CTGF持续递增的表达,提示SGK1在糖尿病肾病肾脏纤维化的发生发展过程中起重要作用。
作者 姜华军 刘建社 朱忠华 王全胜 王玉梅 冯玉锡
机构地区 华中科技大学同济医学院附属协和医院肾内科
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2007年第11期2278-2280,共3页 Chinese Journal of Pathophysiology
基金 国家自然科学基金资助项目(No30270618)
关键词 糖尿病肾病 蛋白激酶类 结缔组织生长因子 Diabetic nephropathies Protein kinases Connective tissure growth factor
分类号 R363 [医药卫生—病理学]
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参考文献7

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共引文献9

同被引文献13

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  • 4Lang F, Henke G, Embark HM, et al . Regulation of channels by the serum and glucocorticoid-inducible kinase-implicatiom for transport, excitability and cell proliferation [ J ]. Cell Physiol Biochem, 2003,13(1) :41-50.
  • 5Buse P, Tran SH, Luther E, et al . Cell cycle and hormonal control of nuclear-cytoplasmic localization of the serum and glucocorticoid-inducible protein kinae, Sgk. in mammary tumor cells. A novel convergence point of anti-proliferative and proliferative cell signaling pathways[J]. J Biol Chem, 1999,274(11) :7253-7263.
  • 6Wang L, Zhou C, Zhu Q, et al . Up-regulation of serum and glucocorticoid-inducible protein kinae 1 in the brain tissue of human and experimental epilepsy[J]. Neurochem Int, 2010, 57(8) : 899-905.
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  • 10张舒静,师水生,田慧,张佑蕊.肝纤维化大鼠SGK1的表达与肝星状细胞活化的关系及坎地沙坦对SGK1的影响[J].临床医药实践,2011,20(7):518-520. 被引量：1

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中国病理生理杂志
2007年 第11期

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