摘要
目的建立速克感冒片中绿原酸和阿司匹林的含量测定方法。方法采用高效液相色谱法,色谱柱C18柱(250 mm×4.6mm,5μm),测定阿司匹林的流动相为甲醇-水-冰醋酸(26∶23∶1)、检测波长为280 nm。测定绿原酸的流动相为甲醇-水-冰醋酸(13∶87∶1)、检测波长为327nm。结果线性范围:阿司匹林30.65~490.4 mg·L^-1(r^2=0.999 6)、绿原酸5.4~108 mg·L^-1(r^2=0.999 9)。加样回收率:阿司匹林为99.55%,RSD为0.38%(n=6);绿原酸为98.78%,RSD为0.56%(n=6)。结论本方法操作简单、结果准确,可作为控制本品质量的方法。
Aim To establish a method for the determination of aspirin and chlorogenic acid in kuaikeganmao tablets.Methods The HPLC method was used.The samples were separated by C18column(250 mm×4.6 mm,5 μm);The Mobile phase of Asprin was Methanol-Water-Acetic acid(26∶ 23∶ 1),and the flow rate was 1.0 ml·min^-1.The detection wavelength was 280 nm;The Mobile phase of chlorogenic acid was Methanol-Water-Acetic acid(13∶ 87∶ 1),and the flow rate was 1.0 ml·min^-1.The detection wavelength was 327 nm.Results The linear range of aspirin was between 30.65 and 490.4 mg·L^-1(r^2=0.999 6) and that of chlorogenic acid was from 5.4 to 108 mg·L^-1(r^2=0.999 9).The average recovery of aspirin was 99.55%,and RSD was 0.38%;and that of chlorogenic acid was 98.78%,and RSD was 0.56%.Conclusion The method is easy,quick,accurate and can be used for quality control of Kuaikeganmao tablet.
出处
《安徽医药》
CAS
2007年第11期1000-1001,共2页
Anhui Medical and Pharmaceutical Journal