VEGF-C对宫颈癌细胞增殖和凋亡影响的研究

VEGF-C effect on the proliferation and apoptosis of Hela cell

目的:探讨VEGF-C对宫颈癌Hela细胞增殖和凋亡的影响。方法:采用MTT比色法,检测不同浓度的VEGF-C对宫颈癌Hela细胞增殖的结果:及采用流式细胞仪检测VEGF-C联合顺铂(DDP)对宫颈癌Hela细胞凋亡的影响。结果:随着VEGF-C的浓度的逐渐升高,由50ng/ml、100ng/ml、150ng/ml、升至200ng/ml,Hela细胞的增殖率逐渐增加;不同浓度的VEGF-C预处理Hela细胞48h后,细胞的凋亡率依次下降。结论:VEGF-C能促进宫颈癌Hela细胞的增殖,抑制DDP诱导宫颈癌细胞Hela的凋亡。

Objective ： To investigate the effect of VEGF-C on the proliferation and apoptosis of Hela cell, Methods： Flow cytometry and MTT assays were used to determined the growth inhabition and Cell cycle, Apoptosis index（AI）after different concentration of human recombinded VEGF-C and DDP. Result; The proliferation of Hela cell transfected by VEGF-C antisense oligonucleotide, measured with MTT assays, was significantly decended. Conclusions： Flow cytometry rneasured, human recombinded VEGF-C can inhibited the apoptosis of Hela cell, indused by DDP .