广西地区汉族人群CD14基因启动子C-260T多态性研究

Study of CD14 gene promoter region C-260T polymorphism in the Hans in Guangxi province

目的:研究CD14基因启动子C-260T多态性各等位基因及基因型在广西地区汉族人群中的分布频率,比较其在不同种族间分布的差异。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测208例汉族人的CD14基因型,并结合文献对不同种族间的CD14基因型和等位基因的分布频率进行比较分析。结果:在广西汉族人群中CC、CT和TT基因型频率分别为32.2%、46.6%和21.2%;等位基因C、T频率分别为55.5%和44.5%。与捷克人比较,CD14基因多态性存在显著性差异。结论:在广西地区汉族人群中存在CD14基因多态性,其在不同种族间的分布可能存在显著性差异,这种差异可能是导致一些疾病在不同种族间的发病率和临床表现存在显著不同的因素之一。

Objective, To study the allele frequencies and genotype distribution of CDI,I gene promoter C-260T polymorphism in the Han ethnic groups in Guangxi province, and to analyze the distributions of CD14 polymorphism were among different races. Methods： The CD14 genotypes were examined by the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） methods in 208 Han populations, frequencies of allele and genotype of CDl4 polymorphism compared with other ethnics. Results： The frequencies of CC, CT and TT genotypes were found to be 32.2%, 46.6% and 21.2% ;the frequencies of C,T allele were found to be 55.5% and 44. 5% in the Hans populations. Compared with czech populations, the CD14 polymorphism was significantly different. Conclusion： There is a CD14 gene promoter C-260T polymorphism in the Hans in Guangxi, whose distribution is significantly different in different ethnic populations. The variation of CD14 gene polymorphism among different ethnic groups might aeeount for the varied clinical spectrum of some CD14 related diseases.